成像模式

参考文献

使用产品

全内反射荧光显微成像

（TIRF）：超高分辨率的单分子可视化；

大视场高分辨HiLo光切片显微成像（HILO）

Whitley, K.D., et al. FtsZ treadmilling is essential for Z-ring condensation and septal constriction initiation in Bacillus subtilis cell 

division. Nat Communications, 2021.

 https://doi.org/10.1038/s41467-021-22526-0

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单颗粒示踪（SPT）

Stuart Middlemiss, et al. Molecular motor tug-of-war regulates elongasome cell wall synthesis dynamics in Bacillus subtilis. bioRxiv, 2023.

https://doi.org/10.1101/2023.05.10.540107

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单颗粒示踪（SPT）

Ranjan A, et al. Live-cell single particle imaging reveals the role of RNA polymerase II in histone H2A.Z eviction. eLife, 2020.

https://pubmed.ncbi.nlm.nih.gov/32338606/

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单颗粒示踪（SPT）

Kim JM, et al. Single-molecule imaging of chromatin remodelers reveals role of ATPase in promoting fast kinetics of target search 

and dissociation from chromatin. Elife, 2021.

https://pubmed.ncbi.nlm.nih.gov/34313223/

单颗粒示踪（SPT）

超分辨显微成像（SIM）

Li Li, et al. Real-time imaging of Huntingtin aggregates diverting target search and gene transcription. eLife, 2016.

https://doi.org/10.7554/eLife.17056

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PA-Janelia Fluor^® 646 HaloTag ligand

超分辨成像（STED）

Wong-Dilworth L, et al. STED imaging of endogenously tagged ARF GTPases reveals their distinct nanoscale localizations. J Cell Biol, 2023.

 https://pubmed.ncbi.nlm.nih.gov/37102998/#

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共聚焦显微成像；

脉冲

 Vevea, J.D., et al. Synaptotagmin 7 is targeted to the axonal plasma membrane through γ-secretase processing to promote synaptic 

vesicle docking in mouse hippocampal neurons. eLife, 2021.

https://elifesciences.org/articles/67261

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共聚焦显微成像；

脉冲

Watson, E.T., et al. Synaptic vesicle proteins are selectively delivered to axons in mammalian neurons. eLife, 2023.

https://elifesciences.org/articles/82568

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单颗粒示踪（SPT）,

常规成像

 Broadbent DG, et al. Quantitative analysis of autophagy reveals the role of ATG9 and ATG2 in autophagosome formation. J Cell Biol. 2023.

https://pubmed.ncbi.nlm.nih.gov/37115157/#

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单颗粒示踪（SPT）

Heyza JR, et al. Systematic analysis of the molecular and biophysical properties of key DNA damage response factors. eLife, 2023.

https://pubmed.ncbi.nlm.nih.gov/37341699/#

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