EcoRI

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Capable of digesting DNA in 15 minutes or less
  • Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
  • Available at high concentration, containing 25,000 units of EcoRI at a concentration of 40–80u/μl
  • Cat.# R6011 is no longer available

Cut Site:

G▼AATT   C
C   TTAA▲G

Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

Note: Star activity may be observed with glycerol concentrations >12%, enzyme:DNA ratios >25u/μg, in the absence of NaCl, in the presence of Mn2+, or under high pH conditions.

Incubation Conditions: Buffer H. 37°C.

Source: Escherichia coli RY 13.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 400mM NaCl, 0.1mM EDTA, 1mM DTT, 0.15% Triton® X-100, 0.5mg/ml BSA, 50% glycerol.

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