Q:Promega有哪些用于细胞信号通路研究的预构建萤光素酶报告基因载体？
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应激信号通路研究萤火虫萤光素酶载体（Stress Signaling Pathway Analysis Response Element Firefly Luciferase Vectors） |
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Activator/Pathway |
Transcription Factor |
Binding Site |
Vector Name |
Catalog Number |
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氧化应激（Oxidative stress） |
Nrf2 |
Antioxidant Response Element (ARE) |
pGL4.37[luc2P/ARE/Hygro] |
E3641 |
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DNA损伤（DNA damage） |
p53 |
p53 response element (p53 RE) |
pGL4.38[luc2P/p53 RE/Hygro] |
E3651 |
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内质网应激（Endoplasmic reticulum stress） |
ATF6 |
Activating Transcription Factor 6 response element (ATF6 ERSE) |
pGL4.39[luc2P/ATF6 RE/Hygro] |
E3661 |
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重金属胁迫（Heavy metal stress） |
MTF1 |
Metal Regulatory Element (MRE) |
pGL4.40[luc2P/MRE/Hygro] |
E4131 |
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热休克（Heat shock） |
HSF1 |
Heat Shock Element (HSE) |
pGL4.41[luc2P/HSE/Hygro] |
E3751 |
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缺氧（Hypoxia） |
Hif1α |
Hypoxia Response Element (HRE) |
pGL4.42[luc2P/HRE/Hygro] |
E4001 |
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外源化合物胁迫（Xenobiotic stress） |
AhR |
Xenobiotic Responsive Element (XRE) |
pGL4.43[luc2P/XRE/Hygro] |
E4121 |
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细胞因子信号通路研究萤火虫萤光素酶载体（Cytokine Signaling Pathway Analysis Response Element Firefly Luciferase Vectors） |
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Activator/Pathway |
Transcription Factor |
Binding Site |
Vector Name |
Catalog Number |
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INF-α |
STAT1:STAT2 |
Interferon Stimulated Response Element (ISRE) |
pGL4.45[luc2P/ISRE/Hygro] |
E4141 |
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TGF-β |
SMAD3:SMAD4 |
SMAD Binding Element (SBE) |
pGL4.48[luc2P/SBE/Hygro] |
E3671 |
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IL6 |
STAT3:STAT3 |
sis-Inducible Element (SIE) |
pGL4.47[luc2P/SIE/Hygro] |
E4041 |
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IL3 |
STAT5:STAT5 |
STAT5 Response Element |
pGL4.52[luc2P/STAT5 RE/Hygro] |
E4651 |
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其他信号通路研究萤火虫萤光素酶载体（Additional Signaling Pathway Analysis Response Element Firefly Luciferase Vectors） |
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Activator/Pathway |
Transcription Factor |
Binding Site |
Vector Name |
Catalog Number |
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MAPK/JNK |
AP1 |
AP1 Response Element (AP1 RE) |
pGL4.44[luc2P/AP1 RE/Hygro] |
E4111 |
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cAMP/PKA |
CREB |
Cyclic AMP Response Element |
pGL4.29[luc2P/CRE/Hygro] |
E8471 |
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NF-κB |
NF-κB |
Nuclear Factor κB Response Element |
pGL4.32[luc2P/NF-κB-RE/Hygro] |
E8491 |
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Calcium/Calcineurin |
NFAT |
Nuclear Factor of Activated T-Cells (NFAT) Response Element |
pGL4.30[luc2P/NFAT-RE/Hygro] |
E8481 |
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MAP/ERK |
Elk-1/SRF |
Serum Response Element |
pGL4.33[luc2P/SRE/Hygro] |
E1340 |
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RhoA |
SRF |
Serum Response Factor Response Element |
pGL4.34[luc2P/SRF-RE/Hygro] |
E1350 |
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Wnt |
TCF-LEF |
TCF-LEF Response Element (TCF-LEF RE) |
pGL4.49[luc2P/TCF-LEF RE/Hygro] |
E4611 |
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信号通路研究NanoLuc®萤光素酶载体（Signaling Pathway Analysis Response Element NanoLuc® Luciferase Vector） |
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Activator/Pathway |
Transcription Factor |
Binding Site |
Vector Name |
Catalog Number |
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NF-κB |
NF-κB |
Nuclear Factor κB Response Element |
pNL3.2.NF-κB-RE[NlucP/NF-κB-RE/Hygro] |
N1111 |
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信号通路研究萤光素酶报告基因定制载体（Custom Luciferase Reporter Vectors for Signaling Pathways） |
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Pathway |
Reporter Gene |
Response Element/Promoter |
Vector Name |
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cAMP/PKA |
NanoLuc® Luciferase |
Cyclic AMP Response Element (CRE) |
pNL[NlucP/CRE/Hygro] Vector |
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Ras/MEK1 |
NanoLuc® Luciferase |
Serum Response Element (SRE) |
pNL[NlucP/SRE/Hygro] Vector |
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RhoA (Gα12/13) |
Firefly Luciferase |
Serum Response Factor (SRF) Response Element |
pGL4[Luc2P/SRF-RE/Puro] Vector |
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NanoLuc® Luciferase |
pNL[NlucP/SRF/Hygro] Vector |
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Calcium/Calcineurin |
Firefly Luciferase |
NFAT Response Element |
pGL4[Luc2P/NFAT-RE/Hygro] Vector |
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NanoLuc® Luciferase |
pNL[NlucP/NFAT-RE/Hygro] Vector |
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Oxidative Stress |
NanoLuc® Luciferase |
Antioxidant Response Element (ARE) |
pNL[NlucP/ARE/Hygro] Vector |
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DNA Damage/p53 |
NanoLuc® Luciferase |
p53 Response Element (p53 RE) |
pNL[NLucP/p53-RE/Hygro] Vector |
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Renilla Luciferase |
pGL4[hRlucP/p53-RE/Hygro] Vector |
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Firefly Luciferase |
pGL4[luc2P/p53-RE/Puro] Vector |
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ER Stress |
Firefly Luciferase |
ATF4 Response Element (ATF4 ERSE) |
pGL4[luc2P/ATF4-RE/Hygro] Vector |
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NanoLuc® Luciferase |
ATF6 Response Element (ATF6 ERSE) |
pNL[NlucP/ATF6-RE/Hygro] Vector |
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NanoLuc® Luciferase |
ATF4 Response Element (ATF4 ERSE) |
pNL[NlucP/ATF4-RE/Hygro] Vector |
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Heavy Metal Stress |
NanoLuc® Luciferase |
Metal Regulatory Element (MRE) |
pNL[NlucP/MRE/Hygro] Vector |
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Hypoxia |
Renilla Luciferase |
Hypoxia Response Element (HRE) |
pGL4[hRlucP/HRE/Hygro] Vector |
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NanoLuc® Luciferase |
pNL[NlucP/HRE/Hygro] Vector |
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Xenobiotic Stress |
NanoLuc® Luciferase |
Xenobiotic Responsive Element (XRE) |
pNL[NlucP/XRE/Hygro] Vector |
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MAPK/JNK |
Renilla Luciferase |
AP1 Response Element (AP1 RE) |
pGL4[hRlucP/AP1-RE/Hygro] Vector |
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Firefly Luciferase |
pGL4[luc2P/AP1-RE/Hygro] Vector |
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NanoLuc® Luciferase |
pNL[NlucP/AP1-RE/Hygro] Vector |
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PI3K/Akt/MAPK |
NanoLuc® Luciferase |
Myc/Max Response Element |
pNL[NlucP/MycMax-RE/Hygro] Vector |
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TGFβ |
NanoLuc® Luciferase |
SMAD3/SMAD4 Binding Element (SBE) |
pNL[NlucP/SBE/Hygro] Vector |
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TGFβ/BMP |
Firefly Luciferase |
Human DNA-Binding Protein Inhibitor ID-1 Promoter |
pGL4[luc2P/hlD1/Hygro] Vector |
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JAK/STAT5 IL3 |
NanoLuc® Luciferase |
STAT5 Response Element |
pNL[NlucP/STAT5-RE/Hygro] Vector |
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JAK/STAT3 IL6 |
NanoLuc® Luciferase |
Sis-Inducible Element (SIE) Response Element |
pNL[NlucP/SIE/Hygro] Vector |
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JAK/STAT1/2 IFNα |
NanoLuc® Luciferase |
Interferon-Stimulated Response Element (ISRE) |
pNL[NlucP/ISRE/Hygro] Vector |
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JAK/STAT4 IL12 |
Firefly Luciferase |
IRF1 Response Element |
pGL4[luc2P/STAT4-RE/Hygro] Vector |
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JAK/STAT1 IFNγ |
Firefly Luciferase |
IFNγ Activation Site (GAS) Response Element |
pGL4[luc2P/GAS-RE/Hygro] Vector |
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NanoLuc® Luciferase |
pNL[NlucP/GAS-RE/Hygro] Vector |
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Wnt |
Firefly Luciferase |
TCF-LEF Response Element (TCF-LEF RE) |
pGL4[luc2CP/TCF-LEF RE/Hygro] Vector |
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NanoLuc® Luciferase |
pNL[NlucP/TCF/LEF-RE/Hygro] Vector |
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Multiple |
Firefly Luciferase |
C/EBP Response Element (C/EBP) |
pGL4[luc2P/C/EBP-RE/Hygro] Vector |
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NanoLuc® Luciferase |
pNL[NlucP/C/EBP-RE/Hygro] Vector |
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Hedgehog |
Firefly Luciferase |
Gli Response Element (Gli) |
pGL4[luc2P/Gli-RE/Hygro] Vector |
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Myc |
Firefly Luciferase |
Myc Response Element (Myc) |
pGL4[luc2P/Myc/Hygro] Vector |
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Notch |
Firefly Luciferase |
CBF1/RBPJk Response Element |
pGL4[luc2P/RBP-Jk-RE/Hygro] Vector |
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IL17 |
Firefly Luciferase |
Human LCN2 (Lipocalin) Promoter |
pGL4[luc2P/LCN2/Hygro] Vector |
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IL1 |
Firefly Luciferase |
Human IL8 Promoter |
pGL4[luc2P/hIL8/Hygro] Vector |
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T Cell Activation IL1 |
Firefly Luciferase |
Human IL2 Promoter |
pGL4[luc2P/IL-2/Hygro] Vector |
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Firefly Luciferase |
pGL4[luc2P/IL-2/Puro] Vector |
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NGF |
Firefly Luciferase |
Human Early Growth Response Factor1 (EGR 1) Promoter |
pGL4[luc2P/hEGR1/Hygro] Vector |
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NFAT |
Firefly Luciferase |
NFAT Response Element |
pGL4[luc2P/NFAT-RE/Puro] Vector |
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Androgen Activation in Prostate Cancer |
Firefly Luciferase |
Human PSA Promoter |
pGL4[luc2P/PSA-long/Hygro] Vector |
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STAT3 |
Firefly Luciferase |
Human G-CSF |
pGL4[luc2P/GCSF/Hygro] Vector |
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Cytochrome 450/Drug Metabolism |
Firefly Luciferase |
Human 2B6 |
pGL4[luc2P/2B6/Hygro] Vector |
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Firefly Luciferase |
Human 3A4 |
pGL4[luc2P/3A4/Hygro] Vector |
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VEGF2 |
Firefly Luciferase |
hVEGFR2 Promoter |
pGL4[Luc2P/hVEGFR2(–290 to +269)/Hygro] Vector |
