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Storage Conditions: For long-term storage, the lyophilized BacTiter-Glo™ Substrate and BacTiter-Glo™ Buffer should be stored at –20°C. For requent use, the BacTiter-Glo™ Buffer can be stored at 4°C or at room temperature for 48 hours without loss of activity. For optimal performance, reconstituted BacTiter-Glo™ Reagent (buffer plus substrate) should be used within eight hours when the reagent is kept at room temperature. The reconstituted BacTiter-Glo™ Reagent can be stored at 4°C for four days, at –20°C for one week or at –70°C for one month with less than 20% loss of activity.