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Storage Conditions: Store the UDP-Glo™ Glycosyltransferase Assay at less than –65°C or store UDP-Glo™ Enzyme at less than –65°C and the other components at –20°C. Before use, completely thaw all components at room temperature except the UDP-Glo™ Enzyme,which should be thawed only prior to use and any remaining volume should be returned to less than –65°C. Once thawed, all components should be thoroughly mixed before use. Any remaining Nucleotide Detection Reagent (Nucleotide Detection Buffer + ATP Detection Substrate) should be dispensed into aliquots and stored at less than –65°C. For best results, prepare only the amount of UDP Detection Reagent(Nucleotide Detection Reagent + UDP-Glo™ working solution) needed. If smaller amounts of UDP Detection Reagent are needed for each use, the UDP-Glo™ Enzyme should be dispensed in single-use aliquots and stored at less than –66°C